Nitrifying Bacteria Contains: Nitrosomonas, Nitrospira and Nitrobactor. Specially Formulated for Rapid Ammonia and Nitrite Reduction Decreases Ammonia & Nitrite Toxicity Treats up to 7,680 gal. (27,072 L.) MICROBE-LIFT/Nite-Out II is designed specifically for aquarium waters that contain marine life. Its highly-specialized microbial consortium of nitrifying cultures are specially formulated to eliminate ammonia via a natural biological process termed nitrification. The cultures contain in MICROBE-LIFT/Nite-Out II will establish, promote or stabilize and maintain nitrification in aquarium waters, eliminating the toxic effect of ammonia. MICROBE-LIFT/Nite-Out II liquid nitrifying bacteria contains select strains of Nitrosomonas, Nitrospira and Nitrobacter. Nitrosomonas oxidize ammonia to nitrite and Nitrobacter and Nitrospira oxidize nitrite to nitrate. Nitrification Nitrosomonas Nitrobacter NH3 => NO2 => NO3 Ammonia Nitrite Nitrate MICROBE-LIFT/Nite-Out II comprises select microorganisms that are autotrophic able to use carbon dioxide as the sole source of carbon and are relatively slow growing, requiring specific conditions for optimum growth with typical cell divisions rates from 8 to 16 hours. Their performance and rate growth is impacted by the environmental parameters required for nitrification. Cautions Keep out of reach of children. Wash hands after use. If eye irritation occurs, flush with water for 15 minutes. Do not take internally. NOTE: Shelf life is 18 months. DON'T FREEZE. Store at room temperature. Avoid sunlight. Refrigerate 38 F (4 C) 45 F (7 C) after opening to prolong shelf life.